New Matsushiro underground cosmic ray station (220 M.W.E. in depth)

A new underground cosmic ray station has been opened at Matsushiro, Japan, and a multidirectional (17 directional channels) muon telescope has been installed at an effective vertical depth of 220 m.w.e. The counting rates are; 8.7 x 10,000/hr for the wide vertical component and 2.0 x 10,000/hr for the vertical component. Continuous observation has been performed since March 22,1984. Some details of the telescope and preliminary analyzed results of the data are presented

BoWFire: Detection of Fire in Still Images by Integrating Pixel Color and Texture Analysis

Emergency events involving fire are potentially harmful, demanding a fast and precise decision making. The use of crowdsourcing image and videos on crisis management systems can aid in these situations by providing more information than verbal/textual descriptions. Due to the usual high volume of data, automatic solutions need to discard non-relevant content without losing relevant information. There are several methods for fire detection on video using color-based models. However, they are not adequate for still image processing, because they can suffer on high false-positive results. These methods also suffer from parameters with little physical meaning, which makes fine tuning a difficult task. In this context, we propose a novel fire detection method for still images that uses classification based on color features combined with texture classification on superpixel regions. Our method uses a reduced number of parameters if compared to previous works, easing the process of fine tuning the method. Results show the effectiveness of our method of reducing false-positives while its precision remains compatible with the state-of-the-art methods.Comment: 8 pages, Proceedings of the 28th SIBGRAPI Conference on Graphics, Patterns and Images, IEEE Pres

Postnatal development of onset transient responses in macaque V1 AND V2 neuron

Vision of newborn infants is limited by immaturities in their visual brain. In adult primates, the transient onset discharges of visual cortical neurons are thought to be intimately involved with capturing the rapid succession of brief images in visual scenes. Here we sought to determine the responsiveness and quality of transient responses in individual neurons of the primary visual cortex (V1) and visual area 2 (V2) of infant monkeys. We show that the transient component of neuronal firing to 640-ms stationary gratings was as robust and as reliable as in adults only 2 wk after birth, whereas the sustained component was more sluggish in infants than in adults. Thus the cortical circuitry supporting onset transient responses is functionally mature near birth, and our findings predict that neonates, known for their impoverished vision, are capable of initiating relatively mature fixating eye movements and of performing in detection of simple objects far better than traditionally though

Use of an artificial miniaturized enzyme in hydrogen peroxide detection by chemiluminescence

Advanced oxidation processes represent a viable alternative in water reclamation for potable reuse. Sensing methods of hydrogen peroxide are, therefore, needed to test both process progress and final quality of the produced water. Several bio-based assays have been developed so far, mainly relying on peroxidase enzymes, which have the advantage of being fast, efficient, reusable, and environmentally safe. However, their production/purification and, most of all, batch-to-batch consistency may inherently prevent their standardization. Here, we provide evidence that a synthetic de novo miniaturized designed heme-enzyme, namely Mimochrome VI*a, can be proficiently used in hydrogen peroxide assays. Furthermore, a fast and automated assay has been developed by using a lab-bench microplate reader. Under the best working conditions, the assay showed a linear response in the 10.0–120 µM range, together with a second linearity range between 120 and 500 µM for higher hydrogen peroxide concentrations. The detection limit was 4.6 µM and quantitation limits for the two datasets were 15.5 and 186 µM, respectively. In perspective, Mimochrome VI*a could be used as an active biological sensing unit in different sensor configurations

A 3D printable adapter for solid-state fluorescence measurements: the case of an immobilized enzymatic bioreceptor for organophosphate pesticides detection

The widespread use of pesticides in the last decades and their accumulation into the environment gave rise to major environmental and human health concerns. To address this topic, the scientific community pointed out the need to develop methodologies to detect and measure the presence of pesticides in different matrices. Biosensors have been recently explored as fast, easy, and sensitive methods for direct organophosphate pesticides monitoring. Thus, the present work aimed at designing and testing a 3D printed adapter useful on different equipment, and a membrane support to immobilize the esterase-2 from Alicyclobacillus acidocaldarius (EST2) bioreceptor. The latter is labelled with the IAEDANS, a bright fluorescent probe. EST2 was selected since it shows a high specificity toward paraoxon. Our results showed good stability and replicability, with an increasing linear fluorescent intensity recorded from 15 to 150 pmol of labelled EST2. Linearity of data was also observed when using the immobilized labelled EST2 to detect increasing amounts of paraoxon, with a limit of detection (LOD) of 0.09 pmol. This LOD value reveals the high sensitivity of our membrane support when mounted on the 3D adapter, comparable to modern methods using robotic workstations. Notably, the use of an independent support significantly simplified the manipulation of the membrane during experimental procedures and enabled it to match the specificities of different systems. In sum, this work emphasizes the advantages of using 3D printed accessories adapted to respond to the newest research needs. Graphical abstract: [Figure not available: see fulltext.

A FRET Approach to Detect Paraoxon among Organophosphate Pesticides Using a Fluorescent Biosensor

The development of faster, sensitive and real-time methods for detecting organophosphate (OP) pesticides is of utmost priority in the in situ monitoring of these widespread compounds. Research on enzyme-based biosensors is increasing, and a promising candidate as a bioreceptor is the thermostable enzyme esterase-2 from Alicyclobacillus acidocaldarius (EST2), with a lipase-like Ser–His–Asp catalytic triad with a high affinity for OPs. This study aimed to evaluate the applicability of Förster resonance energy transfer (FRET) as a sensitive and reliable method to quantify OPs at environmentally relevant concentrations. For this purpose, the previously developed IAEDANS-labelled EST2-S35C mutant was used, in which tryptophan and IAEDANS fluorophores are the donor and the acceptor, respectively. Fluorometric measurements showed linearity with increased EST2-S35C concentrations. No significant interference was observed in the FRET measurements due to changes in the pH of the medium or the addition of other organic components (glucose, ascorbic acid or yeast extract). Fluorescence quenching due to the presence of paraoxon was observed at concentrations as low as 2 nM, which are considered harmful for the ecosystem. These results pave the way for further experiments encompassing more complex matrices

Point-of-care diagnostics of covid-19: From current work to future perspectives

Coronaviruses have received global concern since 2003, when an outbreak caused by SARS‐CoV emerged in China. Later on, in 2012, the Middle‐East respiratory syndrome spread in Saudi Arabia, caused by MERS‐CoV. Currently, the global crisis is caused by the pandemic SARS‐ CoV‐2, which belongs to the same lineage of SARS‐CoV. In response to the urgent need of diagnostic tools, several lab‐based and biosensing techniques have been proposed so far. Five main areas have been individuated and discussed in terms of their strengths and weaknesses. The cell‐culture detection and the microneutralization tests are still considered highly reliable methods. The genetic screening, featuring the well‐established Real‐time polymerase chain reaction (RT‐PCR), represents the gold standard for virus detection in nasopharyngeal swabs. On the other side, immunoassays were developed, either by screening/antigen recognition of IgM/IgG or by detecting the whole virus, in blood and sera. Next, proteomic mass‐spectrometry (MS)‐based methodologies have also been proposed for the analysis of swab samples. Finally, virus-biosensing devices were efficiently designed. Both electrochemical immunosensors and eye‐based technologies have been described, showing detection times lower than 10 min after swab introduction. Alternative to swab‐based techniques, lateral flow point‐of‐care immunoassays are already commercially available for the analysis of blood samples. Such biosensing devices hold the advantage of being portable for on‐site testing in hospitals, airports, and hotspots, virtually without any sample treatment or complicated lab precautions

Receptive-field subfields of V2 neurons in macaque monkey are adult-like near birth

Infant primates can discriminate texture-defined form despite their relatively low visual acuity. The neuronal mechanisms underlying this remarkable visual capacity of infants have not been studied in nonhuman primates. Since many V2 neurons in adult monkeys can extract the local features in complex stimuli that are required for form vision, we used two-dimensional dynamic noise stimuli and local spectral reverse correlation (LSRC) to measure whether the spatial map of receptive-field subfields in individual V2 neurons is sufficiently mature near birth to capture local features. As in adults, most V2 neurons in 4-week-old monkeys showed a relatively high degree of homogeneity in the spatial matrix of facilitatory subfields. However, about 25% of V2 neurons had the subfield map where the neighboring facilitatory subfields substantially differed in their preferred orientations and spatial frequencies. Over 80% of V2 neurons in both infants and adults had ‘tuned’ suppressive profiles in their subfield maps that could alter the tuning properties of facilitatory profiles. The differences in the preferred orientations between facilitatory and suppressive profiles were relatively large but extended over a broad range. Response immaturities in infants were mild; the overall strength of facilitatory subfield responses was lower than that in adults and the optimal correlation delay (‘latency’) was longer in 4-week-old infants. These results suggest that as early as 4 weeks of age, the spatial receptive-field structure of V2 neurons is as complex as in adults and the ability of V2 neurons to compare local features of neighboring stimulus elements is nearly adult like

Dynamics of spatial frequency tuning in mouse visual cortex

Neuronal spatial frequency tuning in primary visual cortex (V1) substantially changes over time. In both primates and cats, a shift of the neuron\u27s preferred spatial frequency has been observed from low frequencies early in the response to higher frequencies later in the response. In most cases, this shift is accompanied by a decreased tuning bandwidth. Recently, the mouse has gained attention as a suitable animal model to study the basic mechanisms of visual information processing, demonstrating similarities in basic neuronal response properties between rodents and highly visual mammals. Here we report the results of extracellular single-unit recordings in the anesthetized mouse where we analyzed the dynamics of spatial frequency tuning in V1 and the lateromedial area LM within the lateral extrastriate area V2L. We used a reverse-correlation technique to demonstrate that, as in monkeys and cats, the preferred spatial frequency of mouse V1 neurons shifted from low to higher frequencies later in the response. However, this was not correlated with a clear selectivity increase or enhanced suppression of responses to low spatial frequencies. These results suggest that the neuronal connections responsible for the temporal shift in spatial frequency tuning may considerably differ between mice and monkeys

Curriculum development for explorative proving in lower secondary school geometry: Focusing on the levels of planning and constructing a proof

This is the final published version. Also available from Frontiers via the DOI in this record.Mathematics education continues to emphasize explorative proving, wherein proving involves producing statements, producing proofs, looking back (examining, improving, and advancing) at these products, and the interactions among these aspects. This study aims to develop an intended explorative proving mathematics curriculum by focusing on students' ability to plan and construct proofs. We first set Levels 1 and 2 of “planning a proof” and “constructing a proof,” respectively, and Level 0 as the starting point of the learning progression where there is no differentiation between planning and constructing. Next, we combined them, and set nine learning levels in addition to “looking back” as the characteristics of explorative proving. Then, we elucidated two learning progressions of explorative proving as a curriculum framework considering the relationship between planning and constructing a proof. To develop the curriculum based on these learning progressions, we made corresponding tables of units with these learning progressions according to the units of Japan's national Course of Study, and then showed an example of localizing one of the progressions and its effects by the implemented curriculum. By adopting the method of lesson study and a design experiment, we implemented geometry lessons for 8th graders that aim to shift the progression through the learning levels. The results clarify the advantages and limitations of the developed curriculum, which enabled us to refine a more robust curriculum. Finally, we identify the characteristics of this approach to curriculum development of explorative proving and the necessity of the method of lesson study and design experiment as a realistic dimension of curriculum development and improvemenGrant-in-Aid for Scientific Research, Ministry of Education, Culture, Sports, Science, and Technology, Japa